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epac1  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Structured Review

    Cell Signaling Technology Inc epac1
    Epac1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epac1/us12502382-67-19-20
    Average 86 stars, based on 1 article reviews
    epac1 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure
    Article Snippet: .. Western blotting was conducted using commercially available antibodies 61 as follows: primary antibodies against myosin phosphatase target subunit 1 (MYPT1) (#612165, BD Transduction Laboratories, 1:1000 dilution), MYPT1/2 (#ab32519, Abcam, 1:2000 dilution), phospho-MYPT (Thr696, #ABS45, Upstate Biotechnology, 1:2000 dilution), Epac1 (#4155, Cell Signaling Technology, 1:1000 dilution), PKAα catalytic subunit (#sc-903, Santa Cruz Biotechnology, 1:200 dilution), PLCε (#ab121476, Abcam, 1:500 dilution), PKC (#12919-1-AP, Proteintech, 1:2000 dilution), CaMKII (#4436, Cell Signaling Technology, 31 1:1000 dilution), cMLCK (#21527-1-AP, Proteintech, 1:1000 dilution), ZIPK (ab210528, Abcam, 1:1000 dilution), histone H3 (#4499, Cell Signaling Technology, 1:2000 dilution), GAPDH (#sc-25778, Santa Cruz Biotechnology, 1:200 dilution), α-actinin (#A7811, Sigma-Aldrich, 1:2500 dilution), phospho-RLC (Ser15, #PA5-104265, Invitrogen, 1:500 dilution), RLC (#10906-1-AP, Proteintech, 1:3000 dilution). .. Horseradish peroxidase-conjugated anti-rabbit IgG (NA934, GE Healthcare) or anti-mouse IgG (NA931, GE Healthcare) was used as a secondary antibody.

    Article Title: Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure
    Article Snippet: .. Western blotting was conducted using commercially available antibodies [ ] as follows: primary antibodies against myosin phosphatase target subunit 1 (MYPT1) (#612165, BD Transduction Laboratories, 1:1000 dilution), MYPT1/2 (#ab32519, Abcam, 1:2000 dilution), phospho-MYPT (Thr696, #ABS45, Upstate Biotechnology, 1:2000 dilution), Epac1 (#4155, Cell Signaling Technology, 1:1000 dilution), PKAα catalytic subunit (#sc-903, Santa Cruz Biotechnology, 1:200 dilution), PLCε (#ab121476, Abcam, 1:500 dilution), PKC (#12919-1-AP, Proteintech, 1:2000 dilution), CaMKII (#4436, Cell Signaling Technology, 1:1000 dilution), cMLCK (#21527-1-AP, Proteintech, 1:1000 dilution), ZIPK (ab210528, Abcam, 1:1000 dilution), histone H3 (#4499, Cell Signaling Technology, 1:2000 dilution), GAPDH (#sc-25778, Santa Cruz Biotechnology, 1:200 dilution), α-actinin (#A7811, Sigma-Aldrich, 1:2500 dilution), phospho-RLC (Ser15, #PA5-104265, Invitrogen, 1:500 dilution), RLC (#10906-1-AP, Proteintech, 1:3000 dilution). .. Horseradish peroxidase-conjugated anti-rabbit IgG (NA934, GE Healthcare) or anti-mouse IgG (NA931, GE Healthcare) was used as a secondary antibody.

    Article Title: Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure.
    Article Snippet: .. Western blotting was conducted using commercially available antibodies [32] as follows: primary antibodies against myosin phosphatase target subunit 1 (MYPT1) (#612165, BD Transduction Laboratories, 1:1000 dilution), MYPT1/2 (#ab32519, Abcam, 1:2000 dilution), phospho-MYPT (Thr696, #ABS45, Upstate Biotechnology, 1:2000 dilution), Epac1 (#4155, Cell Signaling Technology, 1:1000 dilution), PKAα catalytic subunit (#sc-903, Santa Cruz Biotechnology, 1:200 dilution), PLCε (#ab121476, Abcam, 1:500 dilution), PKC (#12919-1-AP, Proteintech, 1:2000 dilution), CaMKII (#4436, Cell Signaling Technology, 1:1000 dilution), cMLCK (#21527-1-AP, Proteintech, 1:1000 dilution), ZIPK (ab210528, Abcam, 1:1000 dilution), histone H3 (#4499, Cell Signaling Technology, 1:2000 dilution), GAPDH (#sc25778, Santa Cruz Biotechnology, 1:200 dilution), α-actinin (#A7811, Sigma-Aldrich, 1:2500 dilution), phospho-RLC (Ser15, #PA5-104265, Invitrogen, 1:500 dilution), RLC (#10906-1-AP, Proteintech, 1:3000 dilution). .. Horseradish peroxidase-conjugated anti-rabbit IgG (NA934, GE Healthcare) or anti-mouse IgG (NA931, GE Healthcare) was used as a secondary antibody.

    Blocking Assay:

    Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue
    Article Snippet: After complete separation of the proteins, they were transferred onto a PVDF membrane (Amersham Biosciences) and were blocked in western blocking buffer (Roche). .. Primary antibodies for EDNRB (NBP130599, Novus Biologicals), UCP1 (ab23841, abcam), phospho-ERK1/2 (Thr202/Tyr204) (#4370, Cell Signaling), total ERK1/2 (#4695, Cell Signaling), phospho-CREB (Ser133) (#9198, Cell Signaling), total CREB (#9197, Cell Signaling), Phospho-PKA Substrate (#9624, Cell Signaling), PPARγ (GTX32803, GeneTex), FABP4/AP2 (GTX636995, GeneTex), EPAC1 (#4155, Cell Signaling), TUBULIN (#2146, Cell Signaling), VINCULIN (sc-25336, Santa Cruz) or ACTIN (MAB1501, Millipore) were applied in blocking buffer over night at 4°C. ..

    Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.
    Article Snippet: After complete separation of the proteins, they were transferred onto a PVDFmembrane (Amersham Biosciences) and were blocked in western blocking buffer (Roche). .. Primary antibodies for EDNRB (NBP130599, Novus Biologicals), UCP1 (ab23841, abcam), phospho-ERK1/2 (Thr202/ Tyr204) (#4370, Cell Signaling), total ERK1/2 (#4695, Cell Signaling), phospho-CREB (Ser133) (#9198, Cell Signaling), total CREB (#9197, Cell Signaling), Phospho-PKA Substrate (#9624, Cell Signaling), PPARγ (GTX32803, GeneTex), FABP4/AP2 (GTX636995, GeneTex), EPAC1 (#4155, Cell Signaling), TUBULIN (#2146, Cell Signaling), VINCULIN (sc25336, Santa Cruz) or ACTIN (MAB1501, Millipore) were applied in blocking buffer over night at 4°C. ..



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    KW6002 prevented mtDNA release by inhibiting <t>EPAC1/VDAC1</t> pathway-mediated opening of MPTP. ( a ) Representative TEM images showing mitochondrial ultrastructure and quantitative analysis of lesions mitochondria in the hippocampal tissues, n = 6/group. ( b ) Cytosolic mtDNA levels of D-loop3, COX1 and mt- ND1 in the hippocampal tissues, n = 4/group. ( c ) Representative images of immunofluorescence staining and quantitative analysis of dsDNA (red)/DAPI (blue), n = 4/group. ( d ) The concentration of cAMP in brain, n = 12/group. ( e , f ) Representative western blot images showing EPAC1 and VDAC1 protein levels in the hippocampal tissues, n = 6/group. ( g ) The levels of inflammatory factors secreted by HY-treated BV2 microglial cells, n = 12/group. ( h ) Representative immunofluorescence staining images showing mitochondrial EPAC1 and VDAC1 levels in HY-induced BV2 cell model, quantitative analysis of levels of EPAC1 and VDAC1, quantitative analysis of colocalization between EPAC1 and VDAC1, n = 8 or 12/group. *** p < 0.001, ** p < 0.01 vs. control group. ### p < 0.001, ## p < 0.01, # p < 0.05 vs. HY group.
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    epac1  (Cell Signaling Technology Inc)
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    KW6002 prevented mtDNA release by inhibiting <t>EPAC1/VDAC1</t> pathway-mediated opening of MPTP. ( a ) Representative TEM images showing mitochondrial ultrastructure and quantitative analysis of lesions mitochondria in the hippocampal tissues, n = 6/group. ( b ) Cytosolic mtDNA levels of D-loop3, COX1 and mt- ND1 in the hippocampal tissues, n = 4/group. ( c ) Representative images of immunofluorescence staining and quantitative analysis of dsDNA (red)/DAPI (blue), n = 4/group. ( d ) The concentration of cAMP in brain, n = 12/group. ( e , f ) Representative western blot images showing EPAC1 and VDAC1 protein levels in the hippocampal tissues, n = 6/group. ( g ) The levels of inflammatory factors secreted by HY-treated BV2 microglial cells, n = 12/group. ( h ) Representative immunofluorescence staining images showing mitochondrial EPAC1 and VDAC1 levels in HY-induced BV2 cell model, quantitative analysis of levels of EPAC1 and VDAC1, quantitative analysis of colocalization between EPAC1 and VDAC1, n = 8 or 12/group. *** p < 0.001, ** p < 0.01 vs. control group. ### p < 0.001, ## p < 0.01, # p < 0.05 vs. HY group.
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    Image Search Results


    KW6002 prevented mtDNA release by inhibiting EPAC1/VDAC1 pathway-mediated opening of MPTP. ( a ) Representative TEM images showing mitochondrial ultrastructure and quantitative analysis of lesions mitochondria in the hippocampal tissues, n = 6/group. ( b ) Cytosolic mtDNA levels of D-loop3, COX1 and mt- ND1 in the hippocampal tissues, n = 4/group. ( c ) Representative images of immunofluorescence staining and quantitative analysis of dsDNA (red)/DAPI (blue), n = 4/group. ( d ) The concentration of cAMP in brain, n = 12/group. ( e , f ) Representative western blot images showing EPAC1 and VDAC1 protein levels in the hippocampal tissues, n = 6/group. ( g ) The levels of inflammatory factors secreted by HY-treated BV2 microglial cells, n = 12/group. ( h ) Representative immunofluorescence staining images showing mitochondrial EPAC1 and VDAC1 levels in HY-induced BV2 cell model, quantitative analysis of levels of EPAC1 and VDAC1, quantitative analysis of colocalization between EPAC1 and VDAC1, n = 8 or 12/group. *** p < 0.001, ** p < 0.01 vs. control group. ### p < 0.001, ## p < 0.01, # p < 0.05 vs. HY group.

    Journal: Scientific Reports

    Article Title: Selective antagonism of adenosine A2A receptor reduces hypobaric hypoxia-induced neuroinflammation by inhibiting cGAS-STING pathway

    doi: 10.1038/s41598-025-30717-8

    Figure Lengend Snippet: KW6002 prevented mtDNA release by inhibiting EPAC1/VDAC1 pathway-mediated opening of MPTP. ( a ) Representative TEM images showing mitochondrial ultrastructure and quantitative analysis of lesions mitochondria in the hippocampal tissues, n = 6/group. ( b ) Cytosolic mtDNA levels of D-loop3, COX1 and mt- ND1 in the hippocampal tissues, n = 4/group. ( c ) Representative images of immunofluorescence staining and quantitative analysis of dsDNA (red)/DAPI (blue), n = 4/group. ( d ) The concentration of cAMP in brain, n = 12/group. ( e , f ) Representative western blot images showing EPAC1 and VDAC1 protein levels in the hippocampal tissues, n = 6/group. ( g ) The levels of inflammatory factors secreted by HY-treated BV2 microglial cells, n = 12/group. ( h ) Representative immunofluorescence staining images showing mitochondrial EPAC1 and VDAC1 levels in HY-induced BV2 cell model, quantitative analysis of levels of EPAC1 and VDAC1, quantitative analysis of colocalization between EPAC1 and VDAC1, n = 8 or 12/group. *** p < 0.001, ** p < 0.01 vs. control group. ### p < 0.001, ## p < 0.01, # p < 0.05 vs. HY group.

    Article Snippet: After blocked with 5% non-fat milk, the membranes were incubated successively with specific primary overnight at 4 °C.and secondary antibodies at room temperature for 1 h. (ADORA1 antibody[ R23384 , 1:1000 dilution, Chengdu Zen-Bioscience], ADORA2A antibody[ab79714, 1:1000 dilution, Abcam], ADORA2B antibody[21071-1-AP, 1:1000 dilution, Proteintech], ADORA3 antibody[820199, 1:1000 dilution, Chengdu Zen-Bioscience], ZO-1 antibody[511417, 1:1000 dilution, Chengdu Zen-Bioscience], Occludin antibody [27260-1-AP, 1:1000 dilution, Proteintech], Claudin 1 antibody[13050-1-AP, 1:1000 dilution, Proteintech], PSD95 antibody[20665-1-AP, 1:1000 dilution, Proteintech], SYN1 antibody[20258-1-AP, 1:1000 dilution, Proteintech], ARC antibody[16290-1-AP, 1:1000 dilution, Proteintech], BDNF antibody[66292-1-Ig, 1:1000 dilution, Proteintech], HO-1 antibody[ R22808 , 1:1000 dilution, Chengdu Zen-Bioscience], Nrf2 antibody[12721 S, 1:1000 dilution, Cell Signaling Technology], cGAS antibody[31659, 1:1000 dilution, Cell Signaling Technology], STING antibody[19851-1-AP, 1:2000 dilution, Proteintech], p-STING antibody[PA5-105674, 1:500 dilution, Thermo Fisher Scientific], p-IRF3 antibody[530857, 1:1000 dilution, Chengdu Zen-Bioscience], EPAC1 antibody[12572-1-AP, 1:1000 dilution, Proteintech], VDAC1 antibody[ab154856, 1:1000 dilution, Abcam], GAPDH antibody[ R24404 , 1:5000 dilution, Chengdu Zen-Bioscience], ACTB antibody[R380624, 1:5000 dilution, Chengdu Zen-Bioscience], Tubulin antibody[80713-1-RR, 1:1000 dilution, Proteintech], TOM20 antibody[11802-1-AP, 1:5000 dilution, Proteintech]. secondary antibody Goat Anti-Rabbit IgG H&L(HRP)[511203, 1:5000 dilution, Chengdu Zen-Bioscience], secondary antibody Goat Anti-Mouse IgG H&L(HRP)[511103, 1:5000 dilution, Chengdu Zen-Bioscience].

    Techniques: Immunofluorescence, Staining, Concentration Assay, Western Blot, Control

    Schematic illustration depicting that selective antagonism on ADORA2A reduces HY-induced neuroinflammation by inhibiting cGAS-STING pathway. HY exposure enhanced the level of ADORA2A, which in turn promoted the production of cAMP and cascading mitochondrial levels of EPAC1 and VDAC1. The interaction between EPAC1 and VDAC1 regulated MPTP opening and promoted mtDNA release to cytoplasm, which subsequently initiated cGAS-STING inflammatory pathway. Selective antagonism on ADORA2A restores HY-induced neuroinflammation through negatively regulating above biological process. The illustrative picture was created in BioRender. (yehui, G. (2025) https://BioRender.com/e23u690 ).

    Journal: Scientific Reports

    Article Title: Selective antagonism of adenosine A2A receptor reduces hypobaric hypoxia-induced neuroinflammation by inhibiting cGAS-STING pathway

    doi: 10.1038/s41598-025-30717-8

    Figure Lengend Snippet: Schematic illustration depicting that selective antagonism on ADORA2A reduces HY-induced neuroinflammation by inhibiting cGAS-STING pathway. HY exposure enhanced the level of ADORA2A, which in turn promoted the production of cAMP and cascading mitochondrial levels of EPAC1 and VDAC1. The interaction between EPAC1 and VDAC1 regulated MPTP opening and promoted mtDNA release to cytoplasm, which subsequently initiated cGAS-STING inflammatory pathway. Selective antagonism on ADORA2A restores HY-induced neuroinflammation through negatively regulating above biological process. The illustrative picture was created in BioRender. (yehui, G. (2025) https://BioRender.com/e23u690 ).

    Article Snippet: After blocked with 5% non-fat milk, the membranes were incubated successively with specific primary overnight at 4 °C.and secondary antibodies at room temperature for 1 h. (ADORA1 antibody[ R23384 , 1:1000 dilution, Chengdu Zen-Bioscience], ADORA2A antibody[ab79714, 1:1000 dilution, Abcam], ADORA2B antibody[21071-1-AP, 1:1000 dilution, Proteintech], ADORA3 antibody[820199, 1:1000 dilution, Chengdu Zen-Bioscience], ZO-1 antibody[511417, 1:1000 dilution, Chengdu Zen-Bioscience], Occludin antibody [27260-1-AP, 1:1000 dilution, Proteintech], Claudin 1 antibody[13050-1-AP, 1:1000 dilution, Proteintech], PSD95 antibody[20665-1-AP, 1:1000 dilution, Proteintech], SYN1 antibody[20258-1-AP, 1:1000 dilution, Proteintech], ARC antibody[16290-1-AP, 1:1000 dilution, Proteintech], BDNF antibody[66292-1-Ig, 1:1000 dilution, Proteintech], HO-1 antibody[ R22808 , 1:1000 dilution, Chengdu Zen-Bioscience], Nrf2 antibody[12721 S, 1:1000 dilution, Cell Signaling Technology], cGAS antibody[31659, 1:1000 dilution, Cell Signaling Technology], STING antibody[19851-1-AP, 1:2000 dilution, Proteintech], p-STING antibody[PA5-105674, 1:500 dilution, Thermo Fisher Scientific], p-IRF3 antibody[530857, 1:1000 dilution, Chengdu Zen-Bioscience], EPAC1 antibody[12572-1-AP, 1:1000 dilution, Proteintech], VDAC1 antibody[ab154856, 1:1000 dilution, Abcam], GAPDH antibody[ R24404 , 1:5000 dilution, Chengdu Zen-Bioscience], ACTB antibody[R380624, 1:5000 dilution, Chengdu Zen-Bioscience], Tubulin antibody[80713-1-RR, 1:1000 dilution, Proteintech], TOM20 antibody[11802-1-AP, 1:5000 dilution, Proteintech]. secondary antibody Goat Anti-Rabbit IgG H&L(HRP)[511203, 1:5000 dilution, Chengdu Zen-Bioscience], secondary antibody Goat Anti-Mouse IgG H&L(HRP)[511103, 1:5000 dilution, Chengdu Zen-Bioscience].

    Techniques: